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Ligand-biased ensemble receptor docking (LigBEnD): a hybrid ligand/receptor structure-based approach.
Ligand docking to flexible protein molecules can be efficiently carried out through ensemble docking to multiple protein conformations, either from experimental X-ray structures or from in silico simulations. The success of ensemble docking often requires the careful selection of complementary protein conformations, through docking and scoring of known co-crystallized ligands. False positives, in which a ligand in a wrong pose achieves a better docking score than that of native pose, arise as additional protein conformations are added. In the current study, we developed a new ligand-biased ensemble receptor docking method and composite scoring function which combine the use of ligand-based atomic property field (APF) method with receptor structure-based docking. This method helps us to correctly dock 30 out of 36 ligands presented by the D3R docking challenge. For the six mis-docked ligands, the cognate receptor structures prove to be too different from the 40 available experimental Pocketome conformations used for docking and could be identified only by receptor sampling beyond experimentally explored conformational subspace
Understanding the role of chromatin remodeling in the regulation of circadian transcription in Drosophila.
Circadian clocks enable organisms to anticipate daily changes in the environment and coordinate temporal rhythms in physiology and behavior with the 24-h day-night cycle. The robust cycling of circadian gene expression is critical for proper timekeeping, and is regulated by transcription factor binding, RNA polymerase II (RNAPII) recruitment and elongation, and post-transcriptional mechanisms. Recently, it has become clear that dynamic alterations in chromatin landscape at the level of histone posttranslational modification and nucleosome density facilitate rhythms in transcription factor recruitment and RNAPII activity, and are essential for progression through activating and repressive phases of circadian transcription. Here, we discuss the characterization of the BRAHMA (BRM) chromatin-remodeling protein in Drosophila in the context of circadian clock regulation. By dissecting its catalytic vs. non-catalytic activities, we propose a model in which the non-catalytic activity of BRM functions to recruit repressive factors to limit the transcriptional output of CLOCK (CLK) during the active phase of circadian transcription, while the primary function of the ATP-dependent catalytic activity is to tune and prevent over-recruitment of negative regulators by increasing nucleosome density. Finally, we divulge ongoing efforts and investigative directions toward a deeper mechanistic understanding of transcriptional regulation of circadian gene expression at the chromatin level
Direct measurement of penetration length in ultra-thin and/or mesoscopic superconducting structures
We describe a method for direct measurement of the magnetic penetration
length in thin (10 - 100 nm) superconducting structures having overall
dimensions in the range 1 to 100 micrometers. The method is applicable for
broadband magnetic fields from dc to MHz frequencies.Comment: Accepted by Journal of Applied P:hysics (Jun 2006).5 pages, 5 figure
Analysis and control of bifurcation and chaos in averaged queue length in TCP/RED model
This paper studies the bifurcation and chaos phenomena in averaged queue length in a
developed Transmission Control Protocol (TCP) model with Random Early Detection
(RED) mechanism. Bifurcation and chaos phenomena are nonlinear behaviour in network
systems that lead to degradation of the network performance. The TCP/RED model used
is a model validated previously. In our study, only the average queue size k q
−
is
considered, and the results are based on analytical model rather than actual measurements.
The instabilities in the model are studied numerically using the conventional nonlinear
bifurcation analysis. Extending from this bifurcation analysis, a modified RED algorithm
is derived to prevent the observed bifurcation and chaos regardless of the selected
parameters. Our modification is for the simple scenario of a single RED router carrying
only TCP traffic. The algorithm neither compromises the throughput nor the average
queuing delay of the system
Finite Symmetry of Leptonic Mass Matrices
We search for possible symmetries present in the leptonic mixing data from
SU(3) subgroups of order up to 511. Theoretical results based on symmetry are
compared with global fits of experimental data in a chi-squared analysis,
yielding the following results. There is no longer a group that can produce all
the mixing data without a free parameter, but a number of them can accommodate
the first or the second column of the mixing matrix. The only group that fits
the third column is . It predicts and
, in good agreement with experimental results.Comment: Version to appear in Physical Review
Implementing Unitarity in Perturbation Theory
Unitarity cannot be perserved order by order in ordinary perturbation theory
because the constraint UU^\dagger=\1 is nonlinear. However, the corresponding
constraint for , being , is linear so it can be
maintained in every order in a perturbative expansion of . The perturbative
expansion of may be considered as a non-abelian generalization of the
linked-cluster expansion in probability theory and in statistical mechanics,
and possesses similar advantages resulting from separating the short-range
correlations from long-range effects. This point is illustrated in two QCD
examples, in which delicate cancellations encountered in summing Feynman
diagrams of are avoided when they are calculated via the perturbative expansion
of . Applications to other problems are briefly discussed.Comment: to appear in Phys. Rev.
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